2pn embryo

2pn embryo

"PN" is the abbreviation of "pronucleus". Generally, embryologists will observe the fertilization of the eggs on the morning of the second day after the IVF egg retrieval day. At this time, under the microscope, they will see one or many rings appear in the fertilized eggs, which are the "pronuclei". ”

During the test tube cycle, after ovulation induction, when the size of the follicle is guaranteed to be above 18mm, we can inject the egg at night and perform egg retrieval for IVF 34-36 hours later. Generally speaking, after the egg is retrieved, it is placed in the prepared IVF embryo culture medium as soon as possible for incubation, and then fertilized with male sperm after a few hours. Generally speaking, the day of the sperm and egg is considered day 0.

Day 1: In the morning, observe the fertilization status of the eggs retrieved for IVF the day before, that is, whether everything is fertilized normally (whether there are two pronuclei 2PN). According to the number of pronuclei, it becomes NPN (N is the number of pronuclei), and no pronucleus is OPN. The IVF embryos developed from sperm and eggs exceeding 2PN cannot be used and must be discarded.

Day 2: Observe whether the sperm and egg are dividing, how many germ cells there are, 4 germ cells are best, whether there are fragments, no fragments are good, whether they are symmetrical, symmetrical is good.

Day 3: Observe the number of germ cells, 8-10 germ cells are best, and check whether there are any fragments and whether they are symmetrical. A good IVF embryo should be one with 8 germ cells that have divided three times. Sometimes there will be IVF embryos with 4 to 7 germ cells or more than 8 germ cells. If after 3 days of incubation, there are too many IVF embryos to be cultured, the embryos need to be moved to freshly prepared IVF embryo culture medium for further incubation.

Day 4: The embryonic development process progresses to the morula stage.

Day 5: The entire process of embryonic development reaches the test tube embryo stage. At this point, a good IVF embryo should have divided more than 6 times and formed a IVF embryo. By the time the embryo reaches the test tube embryo stage, there are already 75-100 reproductive cells in the test tube baby embryo and the capacity continues to increase. At this time, the test tube embryo includes trophectoderm, blastocyst cavity and inner cell mass. The trophectoderm will produce the test tube embryo, placenta, fetal umbilical cord, and the inner cell mass will produce the fetus.

In most cases, only the healthiest IVF embryos can grow and develop to the 5th day to form IVF embryos. This is also the reason why many sisters have a lot of IVF eggs but only a few can be transplanted IVF embryos. The IVF embryos themselves will undergo a process of survival of the fittest. Sperm deformities, chromosomal abnormalities, etc. will all affect the embryonic development process.

<<:  Internal structure of the uterus

>>:  Endometrial pathology report pictures

Recommend

How to regulate amenorrhea

Amenorrhea puzzles many female friends. Changes i...

How to eliminate breast edema during lactation

The reason why women have breast milk is because ...

What to wear in summer after confinement

Giving birth is an inconvenient thing for women, ...

Want to eat but dare not eat, do you really understand anorexia?

Author: Li Xueni, Chief Physician, Peking Univers...

How long after abortion can I eat spicy food

You should pay special attention to your diet aft...

Can pregnant women take Daknin in 6 months?

During pregnancy, pregnant mothers need to pay at...

Causes of lower abdominal distension and brown vaginal discharge

Many female friends are busy with their work and ...

Light bleeding after 10 days of menstruation

Generally speaking, women are not likely to have ...

How to prepare fresh daylily before eating? How to eat daylily

Although daylily has high nutritional value, fres...

How to take care after a painless abortion

Nowadays, painless abortion is a popular surgery,...

Women's IVF treatment may affect their life expectancy

The reproductive functions of some women have alw...

What's wrong with the genital ulcer?

Due to their physiological structure, women have ...

What is itchy below a girl?

In fact, I have always paid more attention to the...

How to make food for the elderly How to regulate their daily diet

As the elderly age, their organ functions decline...