Amniocentesis is something that many pregnant women need to do during pregnancy. It is a very routine examination method. In this way, you can know very intuitively whether the fetus in your belly is developing healthily. However, you must be careful after completing this examination. You cannot have sex, and you must also pay attention to the occurrence of colds and fevers to prevent your body from experiencing some side effects. 2. Before puncturing the amniotic fluid, the pregnant woman can lie in bed and turn over five times in a row to suspend the fetal cells in the amniotic fluid in the uterus, making it easier to puncture and extract more fetal cells. 3. Use the puncture kit to disinfect the puncture site according to aseptic requirements, spread the hole towel, puncture with the puncture needle, slowly extract 10-20 ml of amniotic fluid, slowly inject it into the sterile centrifuge tube along the tube wall, and seal it with a sterile seal; 4. Centrifuge conventionally, open the seal under sterile conditions, discard the supernatant, keep about 1 ml of amniotic fluid and mix the cell sediment into a cell suspension for genetic testing or cell culture; 5. Pour 1 ml of the cell suspension used for genetic testing into the prepared 1.5 ml sterile sealed plastic centrifuge bottle; seal it together with the detailed test records of the pregnant woman, put it in an ice pack and send it to the testing unit (or send it by express mail). The testing unit will keep the file after receiving it and perform genetic testing (chargeable item). 6. For cell culture, evenly distribute the suspension into the prepared culture bottles, seal them aseptically and place them in a CO2 incubator for culture. Observe the growth with an inverted microscope every few days and replace part of the culture medium as needed. After culturing for 15-20 days, when the cells cover the wall of the bottle, harvest the cells for chromosome analysis. Life Details 1. No sexual intercourse is allowed three days before the operation; please take a shower one day before the operation; please empty your bladder 10 minutes before the operation. 2. If you have any abnormalities such as cold, fever, skin infection, etc. 3 to 7 days before the operation, please inform the doctor during the preoperative registration. 3. If you have a history of allergies, special diseases, or other conditions that require explanation, please inform your doctor during preoperative registration. 4. You can go home after sitting and resting for at least 2 hours after the operation; it is best for out-of-town patients to stay in the city that night. 5. Do not take a bath within 24 hours after the operation. Pay more attention to rest and avoid excessive exercise and physical labor such as lifting heavy objects. 6. Sexual intercourse is prohibited for half a month after the operation. 7. If you experience abdominal pain, bloating, vaginal discharge, bleeding, fever, etc. within three days after the operation, these are signs that your pregnancy is in a dangerous situation. Please go to the obstetrics and gynecology department of the hospital as soon as possible. Life care Please arrive at the hospital one hour in advance after lunch to rest and wait, and please be accompanied by a family member. |
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