Amniocentesis in pregnant women

When the baby's due date is approaching, what everyone is most concerned about is when the baby can be born. At this time, everyone pays attention to the issue of amniotic fluid. We often hear that if the amniotic fluid breaks, it means that the baby is about to be born. This is very familiar to everyone, but how much do you know about amniocentesis? Below I will introduce to you what are the examination methods for amniocentesis in pregnant women? I hope this can help everyone.

Amniocentesis is a method of prenatal diagnosis. Generally suitable for prenatal diagnosis in mid-pregnancy. Amniotic fluid exists in the amniotic cavity; the fertilized egg forms the amniotic cavity on the seventh day after fertilization and begins to produce amniotic fluid. The amount of amniotic fluid is 50 ml at 12 weeks of pregnancy, 400 ml at 20 weeks, and 1000-1500 ml at 36-38 weeks. The amount of amniotic fluid decreases slightly as the expected date of delivery approaches.

Amniocentesis for pregnant women:

1. For pregnant women in the appropriate period of pregnancy, use B-ultrasound to determine the abdominal puncture point (where there is a lot of amniotic fluid and no fetal limbs), or perform puncture under B-ultrasound monitoring;

2. Before puncturing the amniotic fluid, the pregnant woman can lie in bed and turn over five times in a row to suspend the fetal cells in the amniotic fluid in the uterus, making it easier to puncture and extract more fetal cells.

3. Use the puncture kit to disinfect the puncture site according to aseptic requirements, spread the hole towel, puncture with the puncture needle, slowly extract 10-20 ml of amniotic fluid, slowly inject it into the sterile centrifuge tube along the tube wall, and seal it with a sterile seal;

4. Centrifuge conventionally, open the seal under sterile conditions, discard the supernatant, keep about 1 ml of amniotic fluid and mix the cell sediment into a cell suspension for genetic testing or cell culture;

5. Pour 1 ml of the cell suspension used for genetic testing into the prepared 1.5 ml sterile sealed plastic centrifuge bottle; seal it together with the detailed test records of the pregnant woman, put it in an ice pack and send it to the testing unit (or send it by express mail). The testing unit will keep the file after receiving it and perform genetic testing (chargeable item).

6. For cell culture, evenly distribute the suspension into the prepared culture bottles, seal them aseptically and place them in a CO2 incubator for culture. Observe the growth with an inverted microscope every few days and replace part of the culture medium as needed. After culturing for 15-20 days, when the cells cover the wall of the bottle, harvest the cells for chromosome analysis.

The above is the content of the examination method of amniocentesis for pregnant women. Everyone should pay more attention to this knowledge in daily life. I will stop here for now. If you still have any questions, you can follow me at any time. I will continue to serve you wholeheartedly.